Geoffrey D Keeler

Geoffrey D Keeler,

Research Assistant Professor

Department: MD-PEDS-CELL / MOLEC THERP DIV
Business Phone: (352) 273-8258
Business Email: gkeeler@ufl.edu

Teaching Profile

Courses Taught
2020-2022,2024
GMS6253 Molecular Therapy III – Immunology of Gene Transfer
2024
GMS6252 Molecular Therapy II – Disease Targets and Applications

Research Profile

Open Researcher and Contributor ID (ORCID)

0000-0002-1517-8894

Publications

2024
Enhanced transgene expression from single-stranded AAV vectors in human cells in vitro and in murine hepatocytes in vivo.
Molecular therapy. Nucleic acids. 35(2) [DOI] 10.1016/j.omtn.2024.102196. [PMID] 38766527.
2024
Upregulation of CD8+ regulatory T cells following liver-directed AAV gene therapy
Cellular Immunology. 397-398 [DOI] 10.1016/j.cellimm.2024.104806.
2023
Development of capsid- and genome-modified optimized AAVrh74 vectors for muscle gene therapy.
Molecular therapy. Methods & clinical development. 31 [DOI] 10.1016/j.omtm.2023.101147. [PMID] 38046199.
2023
Induction of antigen-specific tolerance by hepatic AAV immunotherapy regardless of T cell epitope usage or mouse strain background.
Molecular therapy. Methods & clinical development. 28:177-189 [DOI] 10.1016/j.omtm.2022.12.011. [PMID] 36700122.
2023
Rational Design of AAV-rh74, AAV3B, and AAV8 with Limited Liver Targeting
Viruses. 15(11) [DOI] 10.3390/v15112168. [PMID] 38005848.
2021
AAV3-miRNA vectors for growth suppression of human hepatocellular carcinoma cells in vitro and human liver tumors in a murine xenograft model in vivo.
Gene therapy. 28(7-8):422-434 [DOI] 10.1038/s41434-020-0140-1. [PMID] 32152434.
2020
Enhanced Transduction of Human Hematopoietic Stem Cells by AAV6 Vectors: Implications in Gene Therapy and Genome Editing.
Molecular therapy. Nucleic acids. 20:451-458 [DOI] 10.1016/j.omtn.2020.03.009. [PMID] 32276210.
2020
Type I IFN Sensing by cDCs and CD4+ T Cell Help Are Both Requisite for Cross-Priming of AAV Capsid-Specific CD8+ T Cells.
Molecular therapy : the journal of the American Society of Gene Therapy. 28(3):758-770 [DOI] 10.1016/j.ymthe.2019.11.011. [PMID] 31780366.
2019
Liver induced transgene tolerance with AAV vectors.
Cellular immunology. 342 [DOI] 10.1016/j.cellimm.2017.12.002. [PMID] 29576315.
2018
Gene Therapy-Induced Antigen-Specific Tregs Inhibit Neuro-inflammation and Reverse Disease in a Mouse Model of Multiple Sclerosis.
Molecular therapy : the journal of the American Society of Gene Therapy. 26(1):173-183 [DOI] 10.1016/j.ymthe.2017.09.001. [PMID] 28943274.
2016
Superior In vivo Transduction of Human Hepatocytes Using Engineered AAV3 Capsid.
Molecular therapy : the journal of the American Society of Gene Therapy. 24(6):1042-1049 [DOI] 10.1038/mt.2016.61. [PMID] 27019999.
2015
Effects of delayed delivery of dexamethasone-21-phosphate via subcutaneous microdialysis implants on macrophage activation in rats.
Acta biomaterialia. 23:27-37 [DOI] 10.1016/j.actbio.2015.05.011. [PMID] 25985913.
2015
Localized delivery of dexamethasone-21-phosphate via microdialysis implants in rat induces M(GC) macrophage polarization and alters CCL2 concentrations.
Acta biomaterialia. 12:11-20 [DOI] 10.1016/j.actbio.2014.10.022. [PMID] 25449921.
2014
Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 57:60-7 [DOI] 10.1016/j.ejps.2013.11.005. [PMID] 24239995.

Grants

Feb 2023 ACTIVE
Restoring Immune Tolerance in a Model of Multiple Sclerosis
Role: Co-Investigator
Funding: NATL INST OF HLTH NIAID
Dec 2018 – Nov 2019
Identifying optimal CD8 Treg for CAR Treg Therapy
Role: Principal Investigator
Funding: AMERICAN SOCIETY OF GENE AND CELL THERAP

Contact Details

Phones:
Business:
(352) 273-8258
Emails:
Business:
gkeeler@ufl.edu
Addresses:
Business Mailing:
PO Box 100296
GAINESVILLE FL 32610
Business Street:
P.O. Box 100296
Gainesville FL 32610